Genotyping of Rotavirus by Using RT-PCR Methods

Genotyping of Different Pestiviruse Isolates by RT-PCR

1320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in Ag-ELISA assay. All these 39 samples in addition to 5 cytopathic strains were cultured in MD-BK cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 BVDV suspected isolates were detected by RT-PCR using a primer set. Diff...

A Real-Time RT-PCR Assay for Genotyping of Rotavirus Strains

Background: Human rotavirus (HRV) is the causative agent of severe gastroenteritis in children and responsible for two million hospitalizations and more than a half-million deaths annually. Sequence characteristics of the gene segments encoding the VP7 and VP4 proteins are used for the genotype classification of rotavirus. A wide variety of molecular methods are available, mainly based on rever...

Rotavirus Real Time RT-PCR Kit

Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR

Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...

First Molecular Detection and Vp7 (g) Genotyping of Group a Rotavirus by Semi-nested Rt-pcr from Sewage in Nigeria

Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 6000 and dextran was used to concentrate sewage ...